MyBioSource.com's Anti-Rab5 antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot. The Anti-Rab5 antibody was generated using RAB5, and RAB5A as the antigen and it reacts with Human, Mouse, and Rat.
Description
Description: Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: RAB5A(Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. Tuberin functions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis. Ectopically expressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal compartments and stimulated the enlargement of endosomes in cultured cortical neurons. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. RAB5 signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein(GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. RAB5 activation was either continuous or repetitive for up to 10 minutes, but it ended before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation